MUG-Lucifer-met

human (Homo sapiens)

clear cell sarcoma

established in 2019 from the pleural fluid of a 23-year-old woman with clear cell sarcoma of the knee joint after development of thoracic soft-tissue and mediastinal lymph node metastases; patient received pre-treatment with tyrosine kinase inhibitor crizotinib and doxorubicin; sister cell line of the primary tumor is also available (MUG-Lucifer-prim ACC 958)

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A

round, polygonal and spindle-shaped cells growing in suspension and adherently (about 25% in suspension, 25% loosely adherent, 50% adherent); image; image; image; image

90% DMEM/F12 with Glutamax + 10% h.i. FBS

seed out at 0.1-0.4 x 10⁵ cells/cm²; after thawing cells grow slowly for the first few days; split culture 1:2-1:5 every 3-7 days; it is important to collect all (suspended and adherent) cells, use trypsin/EDTA

at 37 °C with 10% CO₂

about 20 hours

0.1-0.15x10⁶ cells/cm² or 10 x10⁶ in a T-75 flask

frozen with 70% medium, 20% FBS, 10% DMSO

negative in PCR assay

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to sister cell line MUG-Lucifer-prim

fusion gene EWSR1::ATF1 was verified by RT-PCR; Reported whole or partial chromosome gains of chromosomes 1, 5, 7, 8, and 17, and partial losses of chromosomes 9 and 15 were confirmed by SNP-array analysis.

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/-2 -, XMLV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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