MUG-CCArly
ACC 960
Cell line:
MUG-CCArly
DSMZ no.:
ACC 960
Species:
human (Homo sapiens)
Cell type:
cholangiocarcinoma
Origin:
established in 2018 from a 72 year old female with untreated bile duct carcinoma (extrahepatic cholangiocellular carcinoma (CCA, grade G2, pT4, Bismuth Corlette type-IV); described to may be cultivated in a 3D co-culture tumor model (CCTM, requires co-culture of this cell line with cancer-associated fibroblast cell line CCArly CAF)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% DMEM/F12 with GlutaMax + 10% FBS
Subculture:
seed out at ca. 2 x 106 cells in a T75 flask; split confluent culture 1:2 to 1:5 twice a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 30-40 hours
Harvest:
cell harvest of ca. 8-12 x 106 cells/75 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/-2 -, XMLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list