YCUB-2
ACC 961
Cell line:
YCUB-2
DSMZ no.:
ACC 961
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
Established in 1997 from a peripheral blood sample of a 4-year old boy with B-cell acute lymphoblastic leukemia (B-ALL) at relapse; cells carry a TCF3::HLF fusion gene deriving from t(17;19) chromosomal translocation
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% FBS
Subculture:
seed out at about 0.5 x 106 cells/ml; split
1:3 to 1:8 every 2-3 days; optimal cell density 0.5-2.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 24 hours
Harvest:
max density of 3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD2 -, CD3 -, CD5 -, CD10 +, CD13 -, CD19 +, CD20 -, CD25 -, CD33 +, CD34 -, CD37 -, CD38 +, CD80 -, CD138 +, HLA-DR +, sm IgG -, sm IgM -, sm/cy kappa -, sm/cy lambda -, cy IgG -, cy IgM +, cy CD79a (+); image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
fusion gene TCF3::HLF was verified by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/-2 -, XMLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list