YCUB-4
ACC 962
Cell line:
YCUB-4
DSMZ no.:
ACC 962
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established in 2006 from peripheral blood of a 7-year-old boy with B-cell precursor ALL (BCP-ALL) at diagnosis; cells express a MEF2D exon5/BCL9 exon 9 fusion; sister cell line YCUB-4R established at relapse is available (DSMZ ACC 963)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% FBS
Subculture:
seed out at about 0.5 x 106 cells/ml; split
1:2 to 1:3 every 2-3 days; we recommend not to exceed a cell density of 1.0 x 106 cells/ml
Doubling time:
about 48 hours
Harvest:
maximal density of ca. 1.5-2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD5 +, CD10 -, CD13 -, CD19 +, CD20 +, CD25 -, CD33 -, CD34 -, CD37 +, CD38 +, CD138 +, HLA-DR +, CD80 -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, matching the sister cell line YCUB-4R
Molec. Genetics:
fusion gene MEF2D::BCL9 was verified by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/-2 -, XMLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list