YCUB-4R
ACC 963
Cell line:
YCUB-4R
DSMZ no.:
ACC 963
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established in 2007 from the peripheral blood of a 7-year-old boy with B-cell precursor acute lymphoblastic leukemia (ALL) at relapse; cells express a MEF2D exon5/BCL9 exon 9 fusion; sister cell line YCUB-4 established at diagnosis is available (DSMZ ACC 962)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% FBS
Subculture:
seed out at about 1 x 106 cells/ml; split
1:2 to 1:3 every 2-3 days; maintain at 0.5-1 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 48 hours
Harvest:
maximal density of ca. 1.5-2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3-, CD5+, CD10(+), CD13-, CD19+, CD20+, CD34-, CD37(+), CD38+, CD80-, CD138(+), HLA-DR+, cyCD79a+, sIgG-, sIgM+, cIgM+, sIg-kappa-, cIg-kappa-; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database matching the sister cell line YCUB-4
Molec. Genetics:
fusion gene MEF2D::BCL9 was verified by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/-2 -, XMLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list