YCUB-5
ACC 964
Cell line:
YCUB-5
DSMZ no.:
ACC 964
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established from the bone marrow of a 12-year-old boy with BCR/ABL1-like (Ph-like) B-cell precursor acute lymphoblastoid leukemia (BCP-ALL) at diagnosis; carries a CRLF2-P2RY8 fusion; JAK2 R683I and KRAS G12D mutations are described
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% FBS
Subculture:
seed out at about 1 x 106 cells/ml; split
1:2 every 3 days; maintain between 0.5-2 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 72 hours
Harvest:
maximal density of ca. 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3-, CD10+, CD13+, CD19+, CD20(+), CD34(+), CD37+, CD38+, CD80-, CD138-, HLA-DR+, sIgG-, sIgM-, sIg-kappa-, sIg-lambda-, cIgG-, cIgM-, cIg-kappa-, cIg-lambda-; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
fusion gene P2RY8::CRLF2 was verified by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/-2 -, XMLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list