M4A1-M2B9
ACC 967
Cell line:
M4A1-M2B9
DSMZ no.:
ACC 967
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established in 2020 from a patient-derived xenograft model (PDX) of a bone marrow sample from a 15-year-old boy with untreated B-cell acute lymphoblastic leukemia; cells carry a MEF2D::BCL9 fusion; transcriptome and exome data are available from the originators upon request (see reference)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS + 1x Glutamax
Subculture:
seed out at about 0.5 x 106 cells/ml; split
1:2 to 1:3 every 2-3 days; maintain between 0.3-0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 48 hours
Harvest:
maximal density of ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 -, CD34 -, CD37 -,
CD38 +, CD80 -, CD138 +, HLA-DR +, cy CD79a +, sm/cy IgG -,
sm/cy IgM +, sm/cy kappa -, sm/cy lambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
fusion gene MEF2D::BCL9 was verified by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/-2 -, XMLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list