BEL-1
ACC 968
Cell line:
BEL-1
DSMZ no.:
ACC 968
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established in 2004 from periphal blood of a 41-year-old-female patient with relapsed B-cell acute lymphoblastic leukemia (B-ALL); cells are described to show a pro B-cell immunophenotype and carry t(4;11)(q21;q23) resulting in KMT2A::AFF1 fusion
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% FBS
Subculture:
seed out at about 0.5 x 106 cells/ml in a 24-well plate; split 1:2 to 1:3 every 2-3 days; maintain at 0.5-1 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 48 hours
Harvest:
max density of 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD5 -, CD10 -, CD13 -, CD19 +, CD20 -, CD33 -, CD37 (+), CD38 +, CD80 -, HLA-DR +, sm kappa +, sm IgG -, sm IgM -, cy IgG +, cy lambda +
; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
fusion gene KMT2A::AFF1 was verified by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/-2 -, XMLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list