H25B10
ACC 98
Cell line:
H25B10
DSMZ no.:
ACC 98
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti hepatitis B)
Origin:
established by fusion of P3-NSI/1-AG4-1 mouse myeloma cells with spleen cells from a mouse immunized with hepatitis B surface antigen; a monoclonal antibody (IgG1) against hepatitis B surface antigen is produced; patented cell line
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
single round to oval cells in suspension
Medium:
90% DMEM (4.5 g/L glucose) + 10% h.i. FBS
Subculture:
cells are difficult to culture! maintain at 0.5-1.0 x 105 cells/ml; split 1:4 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Harvest:
maximal density at about 1-2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Viruses:
ELISA: reverse transcriptase positive
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list