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DetailsCatalogue
Cell line:
CPC-N
DSMZ no.:
ACC 306
Species:
human (Homo sapiens)
Cell type:
small cell lung carcinoma
Origin:
established from the pleural effusion metastasis of a patient with small cell lung carcinoma in 1987 (= CPN)
Reference(s):
14838, 15133
Biosafety level:
1
Permissions and restrictions:
A
 
DSMZ Cell Culture Data:
Morphology:
mostly round cells growing in suspension in very large aggregates preventing exact estimation of cell numbers
Medium:
90% McCoy's 5A + 10% h.i. FBS
Subculture:
split saturated culture 1:2 every 3-4 days; seed out 2 x 106 cells in 5 ml; viability remains at max. 60%, large amounts of cell debris may prevail in the flask
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 4 days
Harvest:
maximal density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
 
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
cytokeratin (+), cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, MDH
Cytogenetics:
human hyperdiploid karyotype with 16% polyploidy - 49(47-51)<2n>XY, +6, +15, +20, add(1)(p36), i(3q), del(6)(q1?3q2?5), der(8)t(8;17)(p23;q21), add(13)(p11)del(13)(q12q22), add(15)(p12), ins(16;8)(p13;HSR?q24) - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form Prices
Frozen culture 360,- €
Growing culture
  (please inquire for exact delivery time)
 720,- €
DNA isolated from cell line (25 µg)  440,- €
DNA isolated from cell line (5 µg)  100,- €

see price list
 
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