Megasphaera elsdenii

DSM 100961

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Name:

Megasphaera elsdenii (Gutierrez et al. 1959) Rogosa 1971

DSM No.:

100961 dsm-100961 dsm100961 dsm 100961 Megasphaera elsdenii

Strain designation:

PG-130-P53-9

Isolated from:

feces; pig, 3 month old, genotype: P53R167H/+ referring to protein p53

Country:

Germany
Kranzberg (Bavaria)

Date of sampling:

01.06.2015

Nagoya Protocol Restrictions:

There are NO known Nagoya Protocol restrictions for this strain.

History:

<- D. Wylensek, TU Munich, Freising-Weihenstephan, Germany <- M. Kornbauer, TU Munich, Freising-Weihenstephan, Germany; PG-130-P53-9

Genbank accession numbers:

16S rRNA gene: MN537448

Cultivation conditions:

Medium 104 , anaerobic, 37°C
or
Medium 339 Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT)., anaerobic, 37°C
or
Medium 693 , anaerobic, 37°C

use liquid medium for reactivation from ampoule

Incubation time: 1-2 days

Complete DSMZ Media List

Summary and
additional information:

<- D. Wylensek, TU Munich, Freising-Weihenstephan, Germany <- M. Kornbauer, TU Munich, Freising-Weihenstephan, Germany; PG-130-P53-9. Feces; pig, 3 month old, genotype: P53R167H/+ referring to protein p53; Germany, Versuchsstation Thalhausen, Kranzberg. Sequence accession no. 16S rRNA gene: MN537448. (Medium 104, 37°C, anaerobic or Medium 339, 37°C, anaerobic or Medium 693, 37°C, anaerobic). use liquid medium for reactivation from ampoule. 01.06.2015, Sampling Date: before 01.07.2015

Risk group:

1 (classification according to German TRBA)

Restrictions:

Commercial use is completely excluded for this strain. For a request for commercial use please contact the depositor: tclavel@ukaachen.de

Supplied as:

Delivery form		Prices

Freeze Dried		120,- €
Active culture on request		280,- €
DNA		180,- €
Price Category for this culture: 1

Freight and handling charges will be added. See price list.

Other cultures:

All DSMZ cultures of the species

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