Summary and
additional information:
<- T. Clavel, RWTH Aachen; CLA-SR-145. Caecal content; wild mouse. Germany, Aachen. Sequence accession no. 16S rRNA gene: OM658603. (Medium 104, incubation time: 2-3 days, gas phase: 100%N2, pH 7, 37°C, anaerobic or Medium 339 Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). For solid medium add 5% horse blood., incubation time: 3-7 days, gas phase: 80%N2, 20%CO2, 37°C). Distribute content of one ampoule (solubilized in 1 ml of liquid medium) on one agar plate.
