Summary and
additional information:
<- J. Ruff <- A.M. Cook, ETH Zurich; PSB-4. Sewage plant material. Taxonomy/description (4550). Plasmid pPSB involved in p-sulfobenzoate degradation (7004). Utilizes 4-sulfobenzoic acid (5175). Please be aware that strains of this species are grouped in Risk Group 2 by German authorities when used in genetic engineering. (Medium 465 (add 2 g/l p-sulfobenzoate from a filter-sterilized stock solution), 30°C or Medium 220, 30°C, rehydrate and grow freeze dried cells from the ampoule in liquid medium 220. Subsequent subculturing may be carried out in liquid medium or on agar 220, or in medium 465).
