DSM No.:
4514 dsm-4514 dsm4514 dsm 4514 pUB110
Other collection no.
or WDCM no.:
ATCC 37015, NCIB 11624
History:
<- ATCC <- D. Dubnau
Remarks:
Natural isolate. Originally discovered in Staphylococcus aureus. General purpose plasmid vector for cloning in Bacillus. Sequence known. Copy number in B. subtilis about 50. The pUB110 replicon is the basis for the construction of chimeric plasmid vectors. The neomycin resistance is expressed in E. coli. Kmr and Neor may be insertionally inactivated within the unique BglII or BanI sites. pUB110 can replicate in other Bacillus species. Transfer of the vector either via transformation or transduction, using transducing phages PBS1, AR9 or SP10.
Distributed in:
Bacillus subtilis subsp. subtilis BD170 (derivative of strain 168)
Risk group:
1 (classification according to German
TRBA)
Country:
country of origin unknown
Date of sampling:
before 16.03.1990
Nagoya Protocol Restrictions:
There are NO known Nagoya protocol restrictions for this strain.
