pYe(CEN3)41

DSM 4932

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PLASMIDS

Name:

pYe(CEN3)41

DSM No.:

4932 dsm-4932 dsm4932 dsm 4932 pYe(CEN3)41

History:

<- J. Carbon

Mol. weight:

9.2 kb

Remarks:

Yeast vector; the plasmid carries CEN3 DNA (centromeric DNA from yeast chromosome III) of Saccharomyces cerevisiae and the replicator ars1 (chromosomal replicator) plus the LEU2 marker; these two markers are located on plasmid pGT12 into which the 2.0 kb CEN3 fragment was cloned; both Tc^r and TRP1 expression are destroyed. The resulting plasmid behaves as a chromosome in mitosis and meiosis in yeast.

Distributed in:

Escherichia coli K12 JA221 C600 hsdR^- hsdM⁺ trpE5 leuB6 recA^-

Cultivation conditions:

Medium 451, 30°C

Literature:

3778

Risk group:

1 (classification according to German TRBA)

Restrictions:

German law regulating genetic engineering / Gentechnikgesetz), Category C

Country:

country of origin unknown