DSM No.:
5593 dsm-5593 dsm5593 dsm 5593 pMOB45
Other collection no.
or WDCM no.:
PC-V3069
Remarks:
Derivative of the runaway-replication plasmid pKN402. General expression E. coli vector with the R1drd19 replicon. Amplifiable to high levels by heat induction: the copy number is then increased (> 100) when cultured at 40°C, roughly 75% of the total DNA is plasmid. This does not lead to inhibition of protein synthesis.
Distributed in:
Escherichia coli K12 DB1443 C600 hsdR
Risk group:
1 (classification according to German
TRBA)
Restrictions:
German law regulating genetic engineering / Gentechnikgesetz),
Category C
Country:
country of origin unknown
Date of sampling:
before 15.06.1990
Nagoya Protocol Restrictions:
There are NO known Nagoya protocol restrictions for this strain.
