DSM No.:
8819 dsm-8819 dsm8819 dsm 8819 pGEc48
History:
<- J. van Beilen, ETH Zürich
Mol. weight:
Approx. 9 kb
Remarks:
The alkBFGH genes have been cloned into the PstI site in pBR322. pGEc48 is a subclone of the 16.9 kb EcoRI fragment which contains the alkBAC operon cloned into pLAFR1. The cloned sequence encodes for a 49-kDa peptide, the largest translation product on the alkBAC operon. The strain is not growing with octane, the plasmid is only selectable with the tetracycline marker. The cloned alk genes are covered by U.S. patent 5,173,428. For general information see pGEc41.
Distributed in:
Escherichia coli K12 GEc137
Risk group:
1 (classification according to German
TRBA)
Restrictions:
German law regulating genetic engineering / Gentechnikgesetz),
Category CMaterial transfer agreement,
Category DCommercial use and transfer to third parties is completely excluded for this strain.
Country:
country of origin unknown
Date of sampling:
before 07.07.1995
Nagoya Protocol Restrictions:
There are NO known Nagoya protocol restrictions for this strain.
