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Bacterial Genomic DNAGenomische DNA von Bakterien

Bacterial Genomic DNA

 

The DSMZ offers quality controlled DNA from nearly all bacterial strains of the collection. Quality is sufficient for PCR-related applications and sequencing on short read platforms such as Illumina. On request, quality controlled DNA can be provided for long read sequencing technologies such as PacBio or Oxford Nanopore Technologies.

If the DNA of a certain strain is available it is listed at the specific catalogue entry of this strain.

For ordering Bacterial Genomic DNA please enter our Shop.

Concentration

PCR assay is tested using 1µl of a 1:10 dilution or 0.5µl of the genomic DNA. Concentrations are delivered on request. Quantity shipped is 25µl.

Methods

Genomic DNAs are isolated from Bacteria and Archaea cultured at DSMZ. For DNA isolation the JETFLEX Genomic DNA Purification Kit from Genomed (Löhne, Germany) or the MasterPure™ Gram Positive DNA Purification Kit from Epicentre (Madison, USA) is used.
Quality of isolated genomic DNAs is controlled by standard 16S rDNA PCR. Authenticity is checked by partial sequencing of the 16S rRNA gene (first ~900 base pairs).

DNAs are stored in TE buffer (10 mM Tris pH 7.5, 1 mM EDTA) at -20°C and are sent at room temperature by airmail. If DNAs shall be sent on dry ice please request. (see Freight Charges).

Your contact partner

Photo of Cathrin  Spröer
Dr. Spröer, Cathrin
Phone: +49-531/2616-101

Partial and complete 16S rRNA gene sequence analysis, phylogenetic analysis, spectroscopic DNA-DNA hybridization